PCR amplification of species specific sequences of 16S rDNA and 16S–23S rDNA intergenic spacer region for identification of Streptococcus phocae
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چکیده
منابع مشابه
Identification of acetic acid bacteria by RFLP of PCR-amplified 16S rDNA and 16S-23S rDNA intergenic spacer.
DNA corresponding to 16S rDNA and the 165-23S rDNA intergenic spacer (ITS) from 22 reference strains of acetic acid bacteria, representing the diversity of the family Acetobacteraceae, and 24 indigenous acetic acid bacteria isolated from wine fermentations were analysed by PCR-RFLP. Frateuria aurantia LMG 1558T and Escherichia coli ATCC 11775T were included as outgroups. PCR-amplified products ...
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Spiroplasma species (Mollicutes: Spiroplasmataceae) are associated with a wide variety of insects, and serology has classified this genus into 34 groups, 3 with subgroups. The 16S rRNA gene has been used for phylogenetic analysis of spiroplasmas, but this approach is uninformative for group VIII because the serologically distinct subgroups generally have similarity coefficients >0.990. Therefor...
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The aim of the current study was to develop a molecular system for differential identification of various fish diseases caused by Flavobacterium species. The system uses the hypervariability of the 16S-23S rDNA Intergenic spacer region (ISR) to develop PCR-based sequence analysis assay. For this purpose, eight different 16S-23S rDNA ISR sequences of six different Flavobacterium species were ali...
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ژورنال
عنوان ژورنال: Microbiological Research
سال: 2008
ISSN: 0944-5013
DOI: 10.1016/j.micres.2006.01.017